APC-Cy^®7 Conjugated Antibodies

Choose APC-Cy7 antibody conjugates to enhance multicolor flow cytometry and immunophenotyping workflows. The far-red/NIR emission (~785 nm), high brightness, and sensitivity labels low-abundance markers while minimizing background autofluorescence and spectral overlap. Easily excited by 633 or 640 nm lasers, APC-Cy^®7 is ideal for panels requiring precise detection and minimal interference.

Flow cytometric analysis of live mouse peritoneal macrophages using F4/80 (BM8.1) Rat mAb (APC-Cy7 Conjugate) #19233 (right), compared to concentration-matched Rat (LTF-2) mAb IgG2b Isotype Control (APC-Cy^®7 Conjugate) #63801 (left)

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Attributes

The excitation spectrum (dashed red line) and emission spectrum (solid red) for APC-Cy®7 are shown above. This tandem fluorophore combines APC’s brightness with the Cy7 far-red emission to minimize spectral overlap and reduce background autofluorescence. Its large Stokes shift and compatibility with 633 and 640 nm laser lines make APC-Cy^®7 an ideal addition for multicolor flow cytometry panels.

Applications in Research

APC-Cy^®7 is invaluable in applications that require high sensitivity, clear signal separation, and minimal background interference. It is particularly effective for:

• Targeting highly expressed markers like CD4, CD8, and CD19 for detailed immunophenotyping of immune cell subsets in complex samples such as blood and tissue lysates.
• Multicolor flow cytometry panels, when paired with many of the commonly used blue, green, and orange fluorochromes like Pacific Blue™, FITC, and PE to reduce spectral overlap and compensation needs.
• Sorting specific cell populations based on surface marker expression with minimal crosstalk.

APC-Cy^®7 is widely compatible with diverse detection systems, red laser lines, and filter sets, making it ideal for varied applications such as:

• Flow cytometry and spectral flow cytometry

• Immunophenotyping

• Western blotting

• FACS

Enhance the detection of low abundance targets Flow cytometric analysis of live mouse splenocytes using CD62L/L-Selectin (MEL-14) Rat mAb (APC-Cy®7 Conjugate) #18118 (right) compared to concentration-matched Rat Isotype Control (APC-Cy®7 Conjugate) (left), co-stained with CD44 (IM7) Rat mAb (FITC Conjugate) #75122.

Commitment to Quality

Get accurate and reliable experimental results with functionally validated, ready-to-use APC-Cy^®7 conjugates. Our stringent and reproducible manufacturing process results in high-quality antibody conjugates produced to the same standards you've trusted for over 25 years. For custom or made-to-order conjugates, we rely on our decades of experience, thorough biophysical characterization, and parent antibody validation to deliver a product with high specificity and sensitivity. Every order comes with comprehensive product documentation and access to expert technical support.

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Explore Our APC-Cy^®7 Conjugated Antibodies

CD45R/B220 (RA3-6B2) Rat mAb (APC-Cy7® Conjugate) #31953	F4/80 (BM8.1) Rat mAb (APC-Cy7® Conjugate) #19233	Ly-6G (1A8) Rat mAb (APC-Cy7® Conjugate) #79038

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Related or Alternative Fluorophores