RedFluor 710 Conjugated Antibodies

Reduce background noise in fluorescence imaging with the near-infrared emission of redFluor 710. Excitable by common red lasers and compatible with standard filter sets, redFluor 710 conjugates are highly photostable and easily distinguished from a broad spectrum of fluorophores, including blue, green, yellow, orange, red, and near-infrared, making them ideal for multiplexing experiments.
Confocal immunofluorescent analysis of fixed frozen mouse thymus labeled with CD4 (RM4-5) Rat mAb (redFluor 710 Conjugate) #75508 (red, left) and colabeled with CD45R/B220 (RA3-6B2) Rat mAb (FITC Conjugate) #34399 (green, right) and Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #36618 (cyan pseudocolor, right).

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Attributes

The dashed line represents redFluor 710's absorption spectrum, and the solid line represents its emission spectrum. Multiplexed experiments work best when fluorophores without spectral overlap are selected. While the optimal excitation is near 630 nm, redFluor 710 can also be excited efficiently by a range of red and near-infrared laser lines, including 635 nm and 640 nm. This broad excitation range offers flexibility in experimental setups using different types of red lasers.

Applications in Research

redFluor 710 has a lot to offer researchers due to several key characteristics:

• redFluor 710 can be attached at high molar ratios without significant self-quenching, resulting in brighter conjugates and more sensitive detection.

• High photostability fluorophores are resistant to photobleaching, meaning redFluor 710 can be observed over the course of longer experiments without losing its signal.

• redFluor 710 has minimal non-specific binding to biological molecules, which avoids much of the background fluorescence associated with high non-specific binding. This ensures a clearer image when sensitivity is crucial such as detecting low-abundance proteins or cell populations.

The stability of redFluor 710 makes it compatible with several detection systems and applications, including:

• Flow Cytometry
• Immunofluorescence
• Live in vivo imaging
• Western blotting

Capture Sharp, Well-differentiated Multi-color Images Confocal immunofluorescent analysis of fixed frozen mouse thymus, labeled with CD3 (17A2) Rat mAb (redFluor 710 Conjugate) #26582 (red), Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #20825 (gray pseudocolor), CD45 (D3F8Q) Rabbit mAb (Alexa Fluor® 555 Conjugate) #19581 (green pseudocolor), and DAPI #4083 (blue).

Commitment to Quality

Get accurate and reliable experimental results with functionally validated, ready-to-use redFluor 710 conjugates from CST. Stringent and reproducible manufacturing process results in high-quality antibody conjugates produced to the same standards you've trusted for over 25 years. We rely on our decades of experience, thorough biophysical characterization, and parent antibody validation to deliver a product with high specificity and sensitivity. Every order comes with comprehensive product documentation and access to expert technical support.

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Explore Our redFluor 710 Conjugated Antibodies

Looking for redFluor 710-conjugated primary and secondary antibodies? Browse our wide range of validated products, or use our custom conjugation services to create the perfect tool tailored to your research needs.

CD45 (HI30) Mouse mAb (redFluor 710 Conjugate) #39170	CD45R/B220 (RA3-6B2) Rat mAb (redFluor 710 Conjugate) #82984	Mouse (MOPC-21) mAb IgG1 Isotype Control (redFluor™ 710 Conjugate) #35935

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Related or Alternative Fluorophores