Maximize multiplexing potential in flow cytometry and immunofluorescence workflows with photostable violetFluor 450. Excited by the 405 nm violet laser line, this blue dye has increased water solubility and labeling efficiency and offers reduced background fluorescence.
Confocal immunofluorescent analysis of fixed frozen mouse spleen labeled with CD8α (2.43) Rat mAb (violetFluor 450 Conjugate) #17083 (left, blue) and colabeled with CD3 (17A2) Rat mAb (FITC Conjugate) #86603 (right, green) and DRAQ5 #4084 (right, red).
View violetFluor 450 Conjugated Antibodies
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Excitation: 405 nm |
Emission: 450 nm |
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Laser Line: 405 nm |
Common Filter Set: 450/50 |
The dashed blue line shows the excitation spectrum of violetFluor 450, while the solid line represents its emission spectrum. When selecting compatible antibodies for multiplexing experiments, it is important to avoid emission overlap between antibodies. Optimally excited by the 405 nm violet laser line, violetFluor 450 has an excitation peak quite distinct from its emission peak.
violetFluor 450 conjugates offer several key benefits in scientific research:
The stability of violetFluor 450 conjugates allows them to be utilized in a broad range of experimental applications:
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Reveal Cell Localization with High-PrecisionConfocal immunofluorescent analysis of fixed frozen mouse spleen labeled with CD4 (RM4-5) Rat mAb (violetFluor 450 Conjugate) #92599 (blue, left) and colabeled with CD45R/B220 (RA3-6B2) Rat mAb (FITC Conjugate) #34399 (green, right) and Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #36618 (red, right). |
Get accurate and reliable experimental results with functionally validated, ready-to-use violetFluor 450 conjugates. Our stringent and reproducible manufacturing process results in high-quality antibody conjugates produced to the same standards you've trusted for over 25 years. For custom or made-to-order conjugates, we rely on our decades of experience, thorough biophysical characterization, and parent antibody validation to deliver a product with high specificity and sensitivity. Every order comes with comprehensive product documentation and access to expert technical support.
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View violetFluor 450 Conjugated Antibodies
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Horizon V450 |
ViaFluor405 |
Pacific Blue™ |
For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology, CST, and XP are registered trademarks of Cell Signaling Technology, Inc. Alexa Fluor is a registered trademark, and Pacific Blue is a trademark of Life Technologies Corporation. All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
